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Fig. 8. Inflammation-associated cytosolic signals regulate α7nAChR in human ASM. In non-asthmatic human ASM cells, pharmacological inhibition of p42/44 <t>MAPK</t> (2 μM PD98059; A) <t>or</t> <t>CREB</t> (5 μM 666-15; B) significantly blunted the enhancing effects of TNFα or IL-13 on α7nAChR. CREB inhibition showed specificity for TNFα. *p < 0.05 vs. Vehicle, #p < 0.05 vs. respective inflam matory cytokine. Data represented as mean ± SEM (N = 4).
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Fig. 8. Inflammation-associated cytosolic signals regulate α7nAChR in human ASM. In non-asthmatic human ASM cells, pharmacological inhibition of p42/44 <t>MAPK</t> (2 μM PD98059; A) <t>or</t> <t>CREB</t> (5 μM 666-15; B) significantly blunted the enhancing effects of TNFα or IL-13 on α7nAChR. CREB inhibition showed specificity for TNFα. *p < 0.05 vs. Vehicle, #p < 0.05 vs. respective inflam matory cytokine. Data represented as mean ± SEM (N = 4).
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Fig. 8. Inflammation-associated cytosolic signals regulate α7nAChR in human ASM. In non-asthmatic human ASM cells, pharmacological inhibition of p42/44 MAPK (2 μM PD98059; A) or CREB (5 μM 666-15; B) significantly blunted the enhancing effects of TNFα or IL-13 on α7nAChR. CREB inhibition showed specificity for TNFα. *p < 0.05 vs. Vehicle, #p < 0.05 vs. respective inflam matory cytokine. Data represented as mean ± SEM (N = 4).

Journal: Archives of biochemistry and biophysics

Article Title: Nicotinic α7 acetylcholine receptor (α7nAChR) in human airway smooth muscle.

doi: 10.1016/j.abb.2021.108897

Figure Lengend Snippet: Fig. 8. Inflammation-associated cytosolic signals regulate α7nAChR in human ASM. In non-asthmatic human ASM cells, pharmacological inhibition of p42/44 MAPK (2 μM PD98059; A) or CREB (5 μM 666-15; B) significantly blunted the enhancing effects of TNFα or IL-13 on α7nAChR. CREB inhibition showed specificity for TNFα. *p < 0.05 vs. Vehicle, #p < 0.05 vs. respective inflam matory cytokine. Data represented as mean ± SEM (N = 4).

Article Snippet: STAT3 (SD 1008), AP1 (SR 11302), CREB (666-15) and MAPK (PD 98059) were from Tocris Bioscience (Bristol, UK).

Techniques: Inhibition